Human SPTAN1 (Alpha-Fodrin) CLIA Kit (E-CL-H0987)

Name: Human SPTAN1 (Alpha-Fodrin) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0987
Price: 180.0 USD
Availability: InStock

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Human SPTAN1 (Alpha-Fodrin) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human SPTAN1 in samples.No significant cross-reactivity or interference between Human SPTAN1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	56288.000	63166.000	59727.000	59699
1000	23579.000	25545.000	24562.000	24534
500	11584.000	10854.000	11219.000	11191
250	5503.000	5999.000	5751.000	5723
125	3001.000	2673.000	2837.000	2809
62.5	1439.000	1409.000	1424.000	1396
31.25	649.000	733.000	691.000	663
0	27.000	29.000	28.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	96.490	197.890	674.080	92.880	196.130	734.460
Standard deviation	11.860	15.910	54.330	10.390	21.480	76.970
CV（%）	12.290	8.040	8.060	11.190	10.950	10.480

Recovery

The recovery of Human SPTAN1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	98-116	106
EDTA plasma （n=8）	92-103	97
Cell culture media （n=8）	86-97	91

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human SPTAN1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	87-102	90-103	85-100
1:2	Average	93	96	92
1:4	Range	101-117	87-104	90-102
1:4	Average	109	95	95
1:8	Range	102-114	96-107	92-106
1:8	Average	108	102	99

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SPTAN1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SPTAN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SPTAN1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human SPTAN1. You can calculate the concentration of Human SPTAN1 in the samples by comparing the RLU value of the samples to the standard curve.

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