Human TARC (Thymus Activation Regulated Chemokine) CLIA Kit (E-CL-H0026)

Name: Human TARC (Thymus Activation Regulated Chemokine) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0026
Price: 180.0 USD
Availability: InStock

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Human TARC (Thymus Activation Regulated Chemokine) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	7.5 pg/mL
Detection Range	12.5-800 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human TARC in samples.No significant cross-reactivity or interference between Human TARC and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
800	50735.000	54961.000	52848.000	52820
400	21722.000	25462.000	23592.000	23564
200	10726.000	10592.000	10659.000	10631
100	4259.000	5091.000	4675.000	4647
50	2354.000	2312.000	2333.000	2305
25	1211.000	1091.000	1151.000	1123
12.5	517.000	579.000	548.000	520
0	27.000	29.000	28.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	41.320	106.810	382.440	39.320	109.520	411.930
Standard deviation	3.500	7.880	29.950	3.860	8.620	38.890
CV（%）	8.470	7.380	7.830	9.820	7.870	9.440

Recovery

The recovery of Human TARC was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	92-105	98
EDTA plasma （n=8）	86-99	92
Cell culture media （n=8）	91-108	99

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human TARC and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	97-115	97-111	91-105
1:2	Average	105	103	96
1:4	Range	94-107	91-108	93-106
1:4	Average	101	99	98
1:8	Range	88-99	100-119	98-114
1:8	Average	94	108	105

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TARC. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TARC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TARC, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human TARC. You can calculate the concentration of Human TARC in the samples by comparing the RLU value of the samples to the standard curve.

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