Human TDP43 (Tar DNA Binding Protein 43KDa )CLIA Kit (E-CL-H0642)

Name: Human TDP43 (Tar DNA Binding Protein 43KDa )CLIA Kit
Brand: Elabscience
SKU: E-CL-H0642
Price: 180.0 USD
Availability: InStock

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Human TDP43 (Tar DNA Binding Protein 43KDa )CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human TDP43 in samples.No significant cross-reactivity or interference between Human TDP43 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	61552.000	64792.000	63172.000	63143
2000	26602.000	30632.000	28617.000	28588
1000	15123.000	13485.000	14304.000	14275
500	6946.000	7304.000	7125.000	7096
250	3647.000	3437.000	3542.000	3513
125	1723.000	1641.000	1682.000	1653
62.5	722.000	792.000	757.000	728
0	28.000	30.000	29.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	220.360	635.100	1973.500	219.640	588.140	2132.620
Standard deviation	19.350	50.740	207.610	25.900	54.990	172.960
CV（%）	8.780	7.990	10.520	11.790	9.350	8.110

Recovery

The recovery of Human TDP43 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	89-103	96
EDTA plasma （n=8）	91-104	97
Cell culture media （n=8）	98-112	104

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human TDP43 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	99-114	94-107	102-120
1:2	Average	107	99	110
1:4	Range	100-117	90-104	96-111
1:4	Average	107	95	102
1:8	Range	86-100	96-109	91-106
1:8	Average	93	102	99

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TDP43. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TDP43 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TDP43, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human TDP43. You can calculate the concentration of Human TDP43 in the samples by comparing the RLU value of the samples to the standard curve.

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