Human TK1 (Thymidine Kinase 1, Soluble) CLIA Kit (E-CL-H1437)

Name: Human TK1 (Thymidine Kinase 1, Soluble) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1437
Price: 180.0 USD
Availability: InStock

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Human TK1 (Thymidine Kinase 1, Soluble) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human TK1 in samples.No significant cross-reactivity or interference between Human TK1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	55211.000	61605.000	58408.000	58380
500	21680.000	25728.000	23704.000	23676
250	11099.000	9811.000	10455.000	10427
125	5029.000	5621.000	5325.000	5297
62.5	2708.000	2504.000	2606.000	2578
31.25	1397.000	1343.000	1370.000	1342
15.63	707.000	743.000	725.000	697
0	28.000	28.000	28.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	51.810	133.490	445.010	54.750	141.810	408.200
Standard deviation	5.900	13.400	32.170	6.340	10.950	45.840
CV（%）	11.390	10.040	7.230	11.580	7.720	11.230

Recovery

The recovery of Human TK1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	90-105	97
EDTA plasma （n=8）	99-113	106
Cell culture media （n=8）	98-112	106

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human TK1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	85-97	99-110	99-113
1:2	Average	91	104	107
1:4	Range	100-117	89-100	97-111
1:4	Average	108	94	105
1:8	Range	91-106	93-106	97-110
1:8	Average	97	99	102

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TK1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TK1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TK1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human TK1. You can calculate the concentration of Human TK1 in the samples by comparing the RLU value of the samples to the standard curve.

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