Human TLR9 (Toll-Like Receptor 9) CLIA Kit (E-CL-H0648)

Name: Human TLR9 (Toll-Like Receptor 9) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0648
Price: 180.0 USD
Availability: InStock

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Human TLR9 (Toll-Like Receptor 9) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human TLR9/CD289 in samples.No significant cross-reactivity or interference between Human TLR9/CD289 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	54455.000	61679.000	58067.000	58044
1000	23085.000	25691.000	24388.000	24365
500	11862.000	11150.000	11506.000	11483
250	4878.000	5748.000	5313.000	5290
125	2716.000	2690.000	2703.000	2680
62.5	1347.000	1321.000	1334.000	1311
31.25	699.000	723.000	711.000	688
0	22.000	24.000	23.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	97.900	185.850	900.190	105.070	201.530	909.750
Standard deviation	10.960	18.620	96.140	11.380	14.190	92.430
CV（%）	11.200	10.020	10.680	10.830	7.040	10.160

Recovery

The recovery of Human TLR9 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	94-110	101
EDTA plasma （n=8）	99-117	107
Cell culture media （n=8）	93-108	100

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human TLR9 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	95-110	87-103	87-98
1:2	Average	102	94	93
1:4	Range	87-104	88-103	87-99
1:4	Average	95	95	93
1:8	Range	89-102	91-105	96-109
1:8	Average	96	96	101

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TLR9/CD289. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TLR9/CD289 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TLR9/CD289, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human TLR9/CD289. You can calculate the concentration of Human TLR9/CD289 in the samples by comparing the RLU value of the samples to the standard curve.

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