Human TrxR1 (Thioredoxin Reductase 1) CLIA Kit (E-CL-H1085)

Name: Human TrxR1 (Thioredoxin Reductase 1) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1085
Price: 180.0 USD
Availability: InStock

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Human TrxR1 (Thioredoxin Reductase 1) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human TrxR1 in samples.No significant cross-reactivity or interference between Human TrxR1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	58335.000	61767.000	60051.000	60014
1000	24850.000	26240.000	25545.000	25508
500	12511.000	10457.000	11484.000	11447
250	5215.000	5841.000	5528.000	5491
125	2783.000	2655.000	2719.000	2682
62.5	1421.000	1271.000	1346.000	1309
31.25	676.000	712.000	694.000	657
0	36.000	38.000	37.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	94.970	300.950	929.280	99.800	279.570	841.330
Standard deviation	11.900	35.780	77.320	9.010	21.470	85.560
CV（%）	12.530	11.890	8.320	9.030	7.680	10.170

Recovery

The recovery of Human TrxR1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	88-104	96
EDTA plasma （n=8）	89-102	96
Cell culture media （n=8）	93-106	100

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human TrxR1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	88-99	87-100	92-108
1:2	Average	93	92	99
1:4	Range	96-113	98-114	87-100
1:4	Average	104	106	94
1:8	Range	96-111	94-107	95-111
1:8	Average	104	101	102

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TrxR1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TrxR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TrxR1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human TrxR1. You can calculate the concentration of Human TrxR1 in the samples by comparing the RLU value of the samples to the standard curve.

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