Human UCHL1 (Ubiquitin Carboxyl Terminal Hydrolase L1) CLIA Kit (E-CL-H1382)

Name: Human UCHL1 (Ubiquitin Carboxyl Terminal Hydrolase L1) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1382
Price: 180.0 USD
Availability: InStock

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Human UCHL1 (Ubiquitin Carboxyl Terminal Hydrolase L1) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human UCHL1 in samples.No significant cross-reactivity or interference between Human UCHL1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	56850.000	57720.000	57285.000	57259
500	21417.000	24151.000	22784.000	22758
250	11141.000	10151.000	10646.000	10620
125	5142.000	5210.000	5176.000	5150
62.5	2726.000	2348.000	2537.000	2511
31.25	1363.000	1249.000	1306.000	1280
15.63	722.000	738.000	730.000	704
0	26.000	26.000	26.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	47.880	92.400	378.300	49.470	96.380	373.460
Standard deviation	6.210	7.950	25.270	6.010	11.350	24.720
CV（%）	12.970	8.600	6.680	12.150	11.780	6.620

Recovery

The recovery of Human UCHL1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	91-105	96
EDTA plasma （n=8）	98-114	107
Cell culture media （n=8）	90-101	96

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human UCHL1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	87-99	100-112	87-97
1:2	Average	92	106	92
1:4	Range	101-117	98-111	103-116
1:4	Average	108	105	109
1:8	Range	99-117	90-101	102-114
1:8	Average	107	96	108

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human UCHL1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human UCHL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human UCHL1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human UCHL1. You can calculate the concentration of Human UCHL1 in the samples by comparing the RLU value of the samples to the standard curve.

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