Human VF (Visfatin) CLIA Kit (E-CL-H1105)

Name: Human VF (Visfatin) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1105
Price: 180.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	4.69 pg/mL
Detection Range	7.81-500 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human VF in samples.No significant cross-reactivity or interference between Human VF and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
500	54691.000	57683.000	56187.000	56156
250	22702.000	23186.000	22944.000	22913
125	10157.000	9277.000	9717.000	9686
62.5	4344.000	4714.000	4529.000	4498
31.25	2600.000	2224.000	2412.000	2381
15.63	1352.000	1332.000	1342.000	1311
7.81	727.000	759.000	743.000	712
0	30.000	32.000	31.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	25.850	44.620	180.110	23.300	43.300	177.990
Standard deviation	2.800	5.270	20.420	2.150	4.140	15.910
CV（%）	10.830	11.810	11.340	9.230	9.560	8.940

Recovery

The recovery of Human VF was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	100-114	107
EDTA plasma （n=8）	90-102	97
Cell culture media （n=8）	87-98	92

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human VF and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	98-116	97-111	102-117
1:2	Average	106	105	108
1:4	Range	89-101	84-99	102-115
1:4	Average	95	91	108
1:8	Range	91-105	100-116	93-108
1:8	Average	98	106	99

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human VF. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VF, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human VF. You can calculate the concentration of Human VF in the samples by comparing the RLU value of the samples to the standard curve.

Background