Human VWF (Von Willebrand Factor) CLIA Kit (E-CL-H1288)

Name: Human VWF (Von Willebrand Factor) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1288
Price: 180.0 USD
Availability: InStock

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Human VWF (Von Willebrand Factor) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.19 ng/mL
Detection Range	0.31-20 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human VWF in samples.No significant cross-reactivity or interference between Human VWF and analogues was observed
Recovery	80%-120%
Sample Type	Plasma
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
20	34631.000	35925.000	35278.000	35246
10	12791.000	13779.000	13285.000	13253
5	5717.000	5185.000	5451.000	5419
2.5	2702.000	2734.000	2718.000	2686
1.25	1531.000	1353.000	1442.000	1410
0.63	998.000	900.000	949.000	917
0.31	626.000	714.000	670.000	638
0	31.000	33.000	32.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	1.020	2.470	10.000	0.920	2.440	9.950
Standard deviation	0.100	0.180	0.810	0.110	0.230	0.790
CV（%）	9.800	7.290	8.100	11.960	9.430	7.940

Recovery

The recovery of Human VWF was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	89-104	95
EDTA plasma （n=8）	95-109	103
Cell culture media （n=8）	85-96	90

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human VWF and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	90-101	95-106	97-110
1:2	Average	96	101	103
1:4	Range	93-107	94-108	103-117
1:4	Average	101	100	110
1:8	Range	92-104	97-112	101-114
1:8	Average	99	105	108

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human VWF. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VWF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VWF, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human VWF. You can calculate the concentration of Human VWF in the samples by comparing the RLU value of the samples to the standard curve.

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