Lactate Dehydrogenase (LDH) Activity Assay Kit (WST-8 method) (E-BC-K766-M)
For research use only.
| Detection Principle | Lactate dehydrogenase (LDH) is an oxidoreductase. LDH catalyzes the conversion of lactate to pyruvic acid and back, as it converts NAD+ to NADH and back. LDH is composed of four subunits (tetramer). The two most common subunits are the LDH-M and LDH-H protein. LDH is released into the blood by cells after tissue damage or erythrocyte hemolysis. Extracellular LDH activity is used to detect cell damage or cell death. |
| Synonyms | LDH |
| Sample Type | Serum,Plasma,Animal tissue,Cell culture supernatant |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (450 nm) |
| Research Area | Glycolysis And Lipid Metabolism , Liver And Renal Function , Energy Metabolism |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.11 U/L |
| Detection Range | 0.11-39.9 U/L |
| Precision | inter-assay CV: 2.3% | intra-assay CV: 2.3% |
| Sample Volume | 50 μL |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 10-20 |
| Dog serum | 10-20 |
| Mouse serum | 50-100 |
| Cynomolgus monkey serum | 10-20 |
| 10% Rat spleen tissue homogenate | 150-250 |
| 10% Rat liver tissue homogenate | 250-350 |
| 10% Rat kidney tissue homogenate | 250-350 |
| 10% Rat lung tissue homogenate | 250-350 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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