Maltose Colorimetric Assay Kit (E-BC-K756-M)
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For research use only.
| Detection Principle |
Maltose is a disaccharide formed by two molecules of glucose linked by an α-1, 4 glycosidic bond, also known as maltodisaccharide.Maltose is not only involved in the glucose metabolism in the body, but also widely used in the food and pharmaceutical industries, which is one of the important indicators of product quality control. Maltose is decomposed by enzymes to produce two molecules of glucose. Glucose reacts with the chromogenic agent to form a chromogenic substance, which can show color changes. There is a characteristic absorption peak at 505 nm, and the depth of its color is directly proportional to the content of maltose. |
| Sample Type | Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (500-510 nm, optimum wavelength: 505 nm) |
| Research Area | Plant Physiology Series |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.01 mg/mL |
| Detection Range | 0.01-1.00 mg/mL |
| Precision | inter-assay CV: 2.5-5.0% | intra-assay CV: 1.5-4.5% |
| Sample Volume | 200 μL (tissue homogenate) |
| Assay Time | 2 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Potato tissue homogenate | 2-5 |
| 10% Chinese cabbage leaf tissue homogenate | 2-5 |
| 10% Sweet potato tissue homogenate | 2-5 |
| 10% Corn seed tissue homogenate | 2-5 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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