Micro-Sample

Mini Sample Mouse BAFF (B-cell Activating Factor) ELISA Kit (E-MSEL-M0131)

Name: Mini Sample Mouse BAFF (B-cell Activating Factor) ELISA Kit
Brand: Elabscience
SKU: E-MSEL-M0131
Price: 150.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 510.00	- +
48T	$ 408.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/

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Product Summary

The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	25 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse BAFF in samples.No significant cross-reactivity or interference between BAFF and analogues was observed
Recovery	80%-120%
Sample Type	Serum,plasma,cell supernatant and tissue homogenate
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T*5
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	2.032	2.058	2.045	1.972
2000	1.363	1.333	1.348	1.275
1000	0.943	0.931	0.937	0.864
500	0.465	0.459	0.462	0.389
250	0.261	0.257	0.259	0.186
125	0.155	0.165	0.160	0.087
62.5	0.107	0.950	0.101	0.028
0	0.069	0.077	0.073	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	201.510	470.870	1916.400	182.080	510.510	1943.580
Standard deviation	13.720	29.430	111.730	14.600	45.030	156.850
CV（%）	6.810	6.250	5.830	8.020	8.820	8.070

Recovery

The recovery of Mouse BAFF was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	87-101	94
EDTA plasma （n=8）	87-100	93
Cell culture media （n=8）	94-105	100

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse BAFF and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	86-99	92-103	89-101
1:2	Average	93	97	94
1:4	Range	89-106	88-98	88-102
1:4	Average	96	93	95
1:8	Range	83-96	89-102	87-103
1:8	Average	88	94	94

For the recovery experiment, the samples were spiked with standard solutions of a specific concentration and then serially diluted to 2-fold, 4-fold, 8-fold, and 16-fold dilutions, respectively. The average recovery rate was subsequently calculated.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse BAFF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse BAFF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse BAFF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 5 nm. The OD value is proportional to the concentration of Mouse BAFF. You can calculate the concentration of Mouse BAFF in the samples by comparing the OD of the samples to the standard curve.

Background

BAFF also known as B-cell activating factor or TNFSF13B is a protein important for B-cell survival and maturation. It's a type II transmembrane protein expressed in cells like macrophages and dendritic cells and has a mass of approximately 31 kDa. The BAFF protein is also present on activated T-cells and in the serum as a soluble form. It functions as a cytokine and influences various stages of B-cell development.