Mini Sample Mouse CD117/c-kit (Cluster Of Differentiation 117) ELISA Kit (E-MSEL-M0133)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CD117. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CD117 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CD117, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CD117. You can calculate the concentration of CD117 in the samples by comparing the OD of the samples to the standard curve.

CD117, also termed c-Kit or stem cell factor receptor (SCFR), is a 145 kDa transmembrane tyrosine kinase receptor belonging to the immunoglobulin superfamily and encoded by the c-kit protooncogene. It specifically binds to its ligand stem cell factor (SCF), also known as c-Kit ligand, steel factor or mast cell growth factor. CD117 exhibits broad cell expression patterns, including hematopoietic stem cells, myeloid and erythroid progenitor cells, T and B lymphocyte precursors, mast cells, megakaryocytes, endothelial cells, and multiple embryonic cells such as melanoblasts and primordial germ cells, and is also highly expressed in acute myeloid leukemia (AML) cells. The binding of CD117 to SCF is indispensable for the development and maintenance of hematopoietic stem cells. Additionally, the CD117-SCF signaling axis fulfills vital biological functions in regulating hematopoiesis, gametogenesis and melanogenesis, participating in multiple developmental and cellular homeostasis processes in mice.