Mini Sample Mouse MCP-3 ( Monocyte Chemotactic Protein 3 ) ELISA Kit (E-MSEL-M0101)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MCP-3 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MCP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MCP-3 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of MCP-3 . You can calculate the concentration of MCP-3 in the samples by comparing the OD of the samples to the standard curve.

CCL7, also known as MCP-3, was initially purified from cytokine-stimulated human osteosarcoma cell line (MG-63) conditioned medium. CCL7 has high similarity with CCL2/MCP-1 (71%), CCL8/MCP-2 (58%), and CCL13/MCP-4 (59%) at the amino acid level. Chemokines play a key role in inflammation and, in the initial phase, CXC chemokines chemoattract polymorphonuclear leukocytes (PMNs). The next step in the inflammatory processes is directed in part by CC chemokines, including the monocyte chemoattractant proteins (CCL2, CCL7, CCL8, and CCL13) that chemoattract additional leukocytes subsets.