Monoamine Oxidase (MAO) Activity Fluorometric Assay Kit (E-BC-F093)
For research use only.
| Detection Principle | Monoamine Oxidase (MAO) is widely present in mitochondria of liver, kidney, brain, small intestine and other organ cells, and mainly participates in metabolic inactivation of bioactive amine substances. MAO is divided into two subtypes, MAO-A and MAO-B, based on the binding specificity of substrates or inhibitors, cell distribution, and immune specificity. MAO can catalyze the corresponding substrate to produce H2O2, and H2O2 further forms a red complex with the fluorescent probe under the action of the enzyme. The change of the fluorescence value of the sample at the excitation wavelength of 535 nm and the emission wavelength of 587 nm in unit time can be measured, and the activity of MAO-A and MAO-B of the sample can be detected and calculated by inhibiting it with specific inhibitors. |
| Sample Type | Serum,Plasma,Animal tissue,Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Research Area | Metabolic Diseases , Liver And Renal Function |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.003 U/L |
| Detection Range | 0.003-2.67 U/L |
| Precision | inter-assay CV: 3.4-10.3% | intra-assay CV: 1.5-5.2% |
| Sample Volume | 30 μL |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 2-3 |
| Mouse serum | 2-4 |
| 10% Mouse liver tissue homogenate | 2-4 |
| 10% Mouse kidney tissue homogenate | 2-3 |
| 10% Mouse lung tissue homogenate | 2-3 |
| 10% Mouse heart tissue homogenate | 2-3 |
| 10% Mouse brain tissue homogenate | 1 |
| 10% Shrimp tissue homogenate | 1 |
| 1×10^6 CHO cells | 1 |
| 1×10^6 293T cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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