Mouse AgRP (Agouti Related Protein) CLIA Kit (E-CL-M0706)

Name: Mouse AgRP (Agouti Related Protein) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0706
Price: 180.0 USD
Availability: InStock

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Mouse AgRP (Agouti Related Protein) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	4.69 pg/mL
Detection Range	7.81-500 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse AgRP in samples.No significant cross-reactivity or interference between Mouse AgRP and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
500	53285.000	56813.000	55049.000	55009
250	20810.000	24542.000	22676.000	22636
125	10953.000	9539.000	10246.000	10206
62.5	4508.000	5268.000	4888.000	4848
31.25	2547.000	2415.000	2481.000	2441
15.63	1314.000	1258.000	1286.000	1246
7.81	772.000	780.000	776.000	736
0	39.000	41.000	40.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	24.830	43.640	211.320	23.130	44.350	199.900
Standard deviation	2.790	3.470	24.220	2.100	4.450	13.230
CV（%）	11.240	7.950	11.460	9.080	10.030	6.620

Recovery

The recovery of Mouse AgRP was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	96-113	103
EDTA plasma （n=8）	101-117	108
Cell culture media （n=8）	91-103	96

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse AgRP and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	102-117	98-116	92-107
1:2	Average	108	106	100
1:4	Range	103-118	91-104	97-112
1:4	Average	110	96	104
1:8	Range	93-107	99-116	89-102
1:8	Average	99	107	95

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse AgRP. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse AgRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse AgRP, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse AgRP. You can calculate the concentration of Mouse AgRP in the samples by comparing the RLU value of the samples to the standard curve.

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