Mouse ANGPTL2 (Angiopoietin Like Protein 2) CLIA Kit (E-CL-M0079)

Name: Mouse ANGPTL2 (Angiopoietin Like Protein 2) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0079
Price: 180.0 USD
Availability: InStock

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Mouse ANGPTL2 (Angiopoietin Like Protein 2) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	7.5 pg/mL
Detection Range	12.5-800 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse ANGPTL2 in samples.No significant cross-reactivity or interference between Mouse ANGPTL2 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
800	53678.000	58908.000	56293.000	56253
400	21443.000	23813.000	22628.000	22588
200	10314.000	9818.000	10066.000	10026
100	4728.000	4750.000	4739.000	4699
50	2460.000	2122.000	2291.000	2251
25	1137.000	1121.000	1129.000	1089
12.5	490.000	556.000	523.000	483
0	40.000	40.000	40.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	42.270	118.450	319.940	46.000	109.470	322.580
Standard deviation	5.430	12.630	32.090	5.260	8.530	31.770
CV（%）	12.850	10.660	10.030	11.430	7.790	9.850

Recovery

The recovery of Mouse ANGPTL2 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	93-110	100
EDTA plasma （n=8）	86-99	92
Cell culture media （n=8）	91-103	96

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse ANGPTL2 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	91-103	91-105	95-110
1:2	Average	97	98	103
1:4	Range	96-111	93-107	84-98
1:4	Average	102	99	91
1:8	Range	93-109	93-109	86-97
1:8	Average	100	101	92

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ANGPTL2. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ANGPTL2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ANGPTL2, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse ANGPTL2. You can calculate the concentration of Mouse ANGPTL2 in the samples by comparing the RLU value of the samples to the standard curve.

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