Mouse ANXA5 (Annexin A5) CLIA Kit (E-CL-M0084)

Name: Mouse ANXA5 (Annexin A5) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0084
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse ANXA5 in samples.No significant cross-reactivity or interference between Mouse ANXA5 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	52360.000	60600.000	56480.000	56448
1000	25034.000	26136.000	25585.000	25553
500	11458.000	11318.000	11388.000	11356
250	5142.000	6080.000	5611.000	5579
125	2772.000	2488.000	2630.000	2598
62.5	1336.000	1294.000	1315.000	1283
31.25	679.000	747.000	713.000	681
0	31.000	33.000	32.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	91.900	310.660	684.490	84.230	313.630	741.160
Standard deviation	8.930	29.820	75.980	10.050	29.140	46.840
CV（%）	9.720	9.600	11.100	11.930	9.290	6.320

Recovery

The recovery of Mouse ANXA5 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	100-113	107
EDTA plasma （n=8）	94-107	100
Cell culture media （n=8）	96-110	102

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse ANXA5 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	99-116	92-108	83-95
1:2	Average	106	99	90
1:4	Range	101-114	87-98	101-113
1:4	Average	108	93	107
1:8	Range	95-110	103-115	88-102
1:8	Average	103	109	93

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ANXA5. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ANXA5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ANXA5, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse ANXA5. You can calculate the concentration of Mouse ANXA5 in the samples by comparing the RLU value of the samples to the standard curve.

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