Mouse ApoB (Apolipoprotein B) CLIA Kit (E-CL-M0098)

Name: Mouse ApoB (Apolipoprotein B) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0098
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Mouse ApoB (Apolipoprotein B) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	3.75 ng/mL
Detection Range	6.25-400 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse ApoB in samples.No significant cross-reactivity or interference between Mouse ApoB and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
400	48102.000	52320.000	50211.000	50178
200	18287.000	22231.000	20259.000	20226
100	8792.000	8336.000	8564.000	8531
50	4347.000	4411.000	4379.000	4346
25	2349.000	2179.000	2264.000	2231
12.5	1208.000	1150.000	1179.000	1146
6.25	686.000	696.000	691.000	658
0	32.000	34.000	33.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	20.250	48.760	170.480	22.180	48.930	183.500
Standard deviation	2.090	5.460	17.820	2.550	4.990	14.220
CV（%）	10.320	11.200	10.450	11.500	10.200	7.750

Recovery

The recovery of Mouse ApoB was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	95-107	102
EDTA plasma （n=8）	101-113	107
Cell culture media （n=8）	85-98	91

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse ApoB and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	103-115	100-114	94-111
1:2	Average	109	107	102
1:4	Range	103-116	89-104	104-117
1:4	Average	109	96	110
1:8	Range	90-105	90-104	93-106
1:8	Average	96	96	100

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ApoB. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ApoB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ApoB, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse ApoB. You can calculate the concentration of Mouse ApoB in the samples by comparing the RLU value of the samples to the standard curve.

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