Mouse CASP10 (Caspase 10) CLIA Kit (E-CL-M0176)

Name: Mouse CASP10 (Caspase 10) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0176
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	4.69 pg/mL
Detection Range	7.81-500 pg/mL
Sample Volume	100 μL
Total Assay Time	4 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse CASP10 in samples.No significant cross-reactivity or interference between Mouse CASP10 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
500	51042.000	54258.000	52650.000	52613
250	21215.000	24839.000	23027.000	22990
125	10215.000	9813.000	10014.000	9977
62.5	4722.000	4960.000	4841.000	4804
31.25	2528.000	2372.000	2450.000	2413
15.63	1351.000	1263.000	1307.000	1270
7.81	760.000	772.000	766.000	729
0	35.000	39.000	37.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	26.170	42.500	235.670	28.370	44.710	239.650
Standard deviation	3.090	5.050	26.560	2.710	3.230	14.830
CV（%）	11.810	11.880	11.270	9.550	7.220	6.190

Recovery

The recovery of Mouse CASP10 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	98-113	106
EDTA plasma （n=8）	101-115	108
Cell culture media （n=8）	88-99	94

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse CASP10 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	85-100	101-116	101-118
1:2	Average	92	107	108
1:4	Range	85-98	101-115	85-96
1:4	Average	91	108	91
1:8	Range	97-112	87-99	93-103
1:8	Average	103	93	98

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CASP10. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CASP10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CASP10, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse CASP10. You can calculate the concentration of Mouse CASP10 in the samples by comparing the RLU value of the samples to the standard curve.