Mouse CD6(T-cell differentiation antigen CD6) ELISA Kit (E-EL-M3135)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Cd6. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Cd6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Cd6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse Cd6. You can calculate the concentration of Cd6 in the samples by comparing the OD of the samples to the standard curve.

Mouse CD6 is a type I transmembrane glycoprotein belonging to the group B scavenger receptor cysteine-rich (SRCR) superfamily. This 626-amino-acid murine protein consists of a 24-amino-acid signal sequence, a 372-amino-acid extracellular region with three conserved SRCR domains, and a 204-amino-acid cytoplasmic tail lacking intrinsic catalytic activity. Mouse CD6 shares 70% full-length amino acid identity with its 668-amino-acid human counterpart. It is lowly expressed on immature thymocytes and highly expressed on mature thymocytes, as well as in most peripheral T cells, partial B cells and neuronal cells. CD6 serves as a dual functional adhesion receptor and T cell costimulatory molecule. Its domain 3 binds ALCAM/CD166 to stabilize immunological synapses and modulate thymocyte development, while domain 1 interacts with CD318. It potentiates T cell activation and proliferation via regulating TCR/CD3 signaling. Upon T cell activation, CD6 undergoes multi-site phosphorylation to initiate intracellular signaling, though its downstream pathways remain incompletely defined. It also binds bacterial LPS and LTA to modulate inflammation and acts as a key target for autoimmune and allograft rejection therapies.