Mouse COL4 (Collagen Type Ⅳ) CLIA Kit (E-CL-M0214)

Name: Mouse COL4 (Collagen Type Ⅳ) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0214
Price: 180.0 USD
Availability: InStock

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Mouse COL4 (Collagen Type Ⅳ) CLIA Kit - 1

Mouse COL4 (Collagen Type Ⅳ) CLIA Kit - 2

Mouse COL4 (Collagen Type Ⅳ) CLIA Kit - 3

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.19 ng/mL
Detection Range	0.31-20 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse COL4 in samples.No significant cross-reactivity or interference between Mouse COL4 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
20	31854.000	37044.000	34449.000	34414
10	13585.000	13703.000	13644.000	13609
5	5733.000	4939.000	5336.000	5301
2.5	2493.000	2929.000	2711.000	2676
1.25	1489.000	1403.000	1446.000	1411
0.63	957.000	919.000	938.000	903
0.31	621.000	629.000	625.000	590
0	35.000	35.000	35.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	0.960	1.680	7.010	0.980	1.770	7.170
Standard deviation	0.110	0.150	0.630	0.100	0.170	0.580
CV（%）	11.460	8.930	8.990	10.200	9.600	8.090

Recovery

The recovery of Mouse COL4 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	91-107	99
EDTA plasma （n=8）	86-98	91
Cell culture media （n=8）	90-101	96

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse COL4 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	95-109	89-101	97-114
1:2	Average	101	94	105
1:4	Range	88-101	92-109	91-108
1:4	Average	93	100	98
1:8	Range	96-110	87-101	98-110
1:8	Average	103	92	103

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse COL4. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse COL4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse COL4, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse COL4. You can calculate the concentration of Mouse COL4 in the samples by comparing the RLU value of the samples to the standard curve.

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