Mouse CTGF (Connective Tissue Growth Factor) CLIA Kit (E-CL-M0220)

Name: Mouse CTGF (Connective Tissue Growth Factor) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0220
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Mouse CTGF (Connective Tissue Growth Factor) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse CTGF in samples.No significant cross-reactivity or interference between Mouse CTGF and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	52573.000	62177.000	57375.000	57342
1000	23923.000	26449.000	25186.000	25153
500	12780.000	10998.000	11889.000	11856
250	5086.000	5648.000	5367.000	5334
125	3023.000	2639.000	2831.000	2798
62.5	1366.000	1330.000	1348.000	1315
31.25	665.000	697.000	681.000	648
0	32.000	34.000	33.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	99.130	316.590	841.860	100.520	310.840	821.860
Standard deviation	12.010	22.640	92.270	9.850	28.600	51.280
CV（%）	12.120	7.150	10.960	9.800	9.200	6.240

Recovery

The recovery of Mouse CTGF was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	96-110	104
EDTA plasma （n=8）	86-100	93
Cell culture media （n=8）	87-101	93

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse CTGF and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	99-112	97-110	101-115
1:2	Average	104	104	107
1:4	Range	98-112	102-118	91-102
1:4	Average	104	109	97
1:8	Range	93-105	100-117	95-108
1:8	Average	98	107	101

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CTGF. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CTGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CTGF, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse CTGF. You can calculate the concentration of Mouse CTGF in the samples by comparing the RLU value of the samples to the standard curve.

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