Mouse CXCR3 (CXC-chemokine receptor 3) CLIA Kit (E-CL-M0241)

Name: Mouse CXCR3 (CXC-chemokine receptor 3) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0241
Price: 180.0 USD
Availability: InStock

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Mouse CXCR3 (CXC-chemokine receptor 3) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse CXCR3 in samples.No significant cross-reactivity or interference between Mouse CXCR3 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	58067.000	61041.000	59554.000	59519
1000	24408.000	24796.000	24602.000	24567
500	12000.000	10888.000	11444.000	11409
250	5228.000	5994.000	5611.000	5576
125	2748.000	2680.000	2714.000	2679
62.5	1527.000	1341.000	1434.000	1399
31.25	644.000	724.000	684.000	649
0	35.000	35.000	35.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	105.250	279.680	778.840	98.170	273.460	729.460
Standard deviation	9.100	21.090	65.500	12.070	24.280	60.690
CV（%）	8.650	7.540	8.410	12.290	8.880	8.320

Recovery

The recovery of Mouse CXCR3 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	87-98	93
EDTA plasma （n=8）	90-106	96
Cell culture media （n=8）	93-108	100

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse CXCR3 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	99-114	88-102	97-112
1:2	Average	106	96	103
1:4	Range	97-109	86-100	85-98
1:4	Average	103	91	91
1:8	Range	98-112	86-99	85-99
1:8	Average	104	91	91

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CXCR3. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CXCR3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CXCR3, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse CXCR3. You can calculate the concentration of Mouse CXCR3 in the samples by comparing the RLU value of the samples to the standard curve.

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