Mouse F9 (Coagulation Factor Ⅸ) CLIA Kit (E-CL-M0207)

Name: Mouse F9 (Coagulation Factor Ⅸ) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0207
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Mouse F9 (Coagulation Factor Ⅸ) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse F9 in samples.No significant cross-reactivity or interference between Mouse F9 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	56370.000	64146.000	60258.000	60230
1000	24163.000	26575.000	25369.000	25341
500	12069.000	10823.000	11446.000	11418
250	4957.000	5885.000	5421.000	5393
125	2950.000	2690.000	2820.000	2792
62.5	1471.000	1289.000	1380.000	1352
31.25	688.000	710.000	699.000	671
0	28.000	28.000	28.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	91.700	258.280	824.600	99.060	233.950	752.790
Standard deviation	9.900	21.280	72.810	12.610	25.130	81.530
CV（%）	10.800	8.240	8.830	12.730	10.740	10.830

Recovery

The recovery of Mouse F9 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	90-103	95
EDTA plasma （n=8）	89-101	94
Cell culture media （n=8）	99-113	105

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse F9 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	87-101	91-107	100-115
1:2	Average	94	98	107
1:4	Range	101-116	85-96	98-116
1:4	Average	109	91	106
1:8	Range	89-104	90-106	102-117
1:8	Average	96	97	110

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse F9. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse F9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse F9, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse F9. You can calculate the concentration of Mouse F9 in the samples by comparing the RLU value of the samples to the standard curve.

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