Mouse FSH (follicle-stimulating hormone) CLIA Kit (E-CL-M0308)

Name: Mouse FSH (follicle-stimulating hormone) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0308
Price: 180.0 USD
Availability: InStock

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Mouse FSH (follicle-stimulating hormone) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.19 ng/mL
Detection Range	0.31-20 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse FSH in samples.No significant cross-reactivity or interference between Mouse FSH and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
20	36028.000	36858.000	36443.000	36409
10	13108.000	13892.000	13500.000	13466
5	5516.000	5482.000	5499.000	5465
2.5	2490.000	2572.000	2531.000	2497
1.25	1526.000	1404.000	1465.000	1431
0.63	887.000	859.000	873.000	839
0.31	594.000	666.000	630.000	596
0	34.000	34.000	34.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	0.980	1.710	7.180	0.920	1.720	7.590
Standard deviation	0.120	0.130	0.460	0.100	0.190	0.480
CV（%）	12.240	7.600	6.410	10.870	11.050	6.320

Recovery

The recovery of Mouse FSH was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	88-103	96
EDTA plasma （n=8）	91-106	97
Cell culture media （n=8）	89-104	96

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse FSH and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	95-108	94-108	98-117
1:2	Average	102	99	106
1:4	Range	99-112	99-114	88-102
1:4	Average	105	108	95
1:8	Range	89-100	96-109	100-114
1:8	Average	94	103	107

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse FSH. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FSH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FSH, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse FSH. You can calculate the concentration of Mouse FSH in the samples by comparing the RLU value of the samples to the standard curve.

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