Mouse GFAP (glial fibrillary acidic protein) CLIA Kit (E-CL-M0333)

Name: Mouse GFAP (glial fibrillary acidic protein) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0333
Price: 180.0 USD
Availability: InStock

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Mouse GFAP (glial fibrillary acidic protein) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	4.69 pg/mL
Detection Range	7.81-500 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse GFAP in samples.No significant cross-reactivity or interference between Mouse GFAP and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
500	47487.000	56941.000	52214.000	52182
250	19589.000	23763.000	21676.000	21644
125	10577.000	9781.000	10179.000	10147
62.5	4635.000	5199.000	4917.000	4885
31.25	2382.000	2276.000	2329.000	2297
15.63	1408.000	1280.000	1344.000	1312
7.81	749.000	827.000	788.000	756
0	31.000	33.000	32.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	27.040	70.750	233.920	29.180	72.590	211.360
Standard deviation	3.000	5.540	25.100	2.430	5.360	15.340
CV（%）	11.090	7.830	10.730	8.330	7.380	7.260

Recovery

The recovery of Mouse GFAP was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	87-99	93
EDTA plasma （n=8）	95-109	102
Cell culture media （n=8）	96-114	104

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse GFAP and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	88-102	99-113	92-106
1:2	Average	93	107	99
1:4	Range	100-115	87-103	99-112
1:4	Average	107	95	105
1:8	Range	94-109	101-120	93-108
1:8	Average	102	109	100

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GFAP. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GFAP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GFAP, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse GFAP. You can calculate the concentration of Mouse GFAP in the samples by comparing the RLU value of the samples to the standard curve.

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