Mouse GREM1 (GremLin 1) CLIA Kit (E-CL-M0360)

Name: Mouse GREM1 (GremLin 1) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0360
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse GREM1 in samples.No significant cross-reactivity or interference between Mouse GREM1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	56654.000	61120.000	58887.000	58859
500	21646.000	24256.000	22951.000	22923
250	11095.000	10801.000	10948.000	10920
125	4755.000	5183.000	4969.000	4941
62.5	2825.000	2483.000	2654.000	2626
31.25	1387.000	1221.000	1304.000	1276
15.63	687.000	767.000	727.000	699
0	28.000	28.000	28.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	46.200	147.450	395.850	50.570	137.780	401.370
Standard deviation	5.300	10.370	43.150	4.530	13.190	33.470
CV（%）	11.470	7.030	10.900	8.960	9.570	8.340

Recovery

The recovery of Mouse GREM1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	94-108	101
EDTA plasma （n=8）	92-106	97
Cell culture media （n=8）	96-110	104

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse GREM1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	101-118	88-101	89-103
1:2	Average	109	94	95
1:4	Range	91-106	99-112	86-98
1:4	Average	97	105	92
1:8	Range	88-101	95-109	95-112
1:8	Average	94	101	102

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GREM1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GREM1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GREM1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse GREM1. You can calculate the concentration of Mouse GREM1 in the samples by comparing the RLU value of the samples to the standard curve.

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