Mouse HAase (Hyaluronidase) CLIA Kit (E-CL-M0423)

Name: Mouse HAase (Hyaluronidase) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0423
Price: 180.0 USD
Availability: InStock

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Mouse HAase (Hyaluronidase) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.47 ng/mL
Detection Range	0.78-50 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse HAase in samples.No significant cross-reactivity or interference between Mouse HAase and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
50	35587.000	39075.000	37331.000	37299
25	14562.000	17602.000	16082.000	16050
12.5	7943.000	6799.000	7371.000	7339
6.25	3329.000	3855.000	3592.000	3560
3.13	1889.000	1803.000	1846.000	1814
1.56	1091.000	1045.000	1068.000	1036
0.78	684.000	772.000	728.000	696
0	31.000	33.000	32.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	2.590	7.240	23.050	2.490	7.480	21.050
Standard deviation	0.270	0.510	1.470	0.300	0.640	1.330
CV（%）	10.420	7.040	6.380	12.050	8.560	6.320

Recovery

The recovery of Mouse HAase was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	86-100	93
EDTA plasma （n=8）	100-114	106
Cell culture media （n=8）	91-105	97

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse HAase and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	95-109	98-114	98-111
1:2	Average	103	104	105
1:4	Range	88-102	99-111	87-101
1:4	Average	95	104	93
1:8	Range	92-108	96-110	95-110
1:8	Average	99	103	100

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse HAase. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HAase and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HAase, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse HAase. You can calculate the concentration of Mouse HAase in the samples by comparing the RLU value of the samples to the standard curve.

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