Mouse HHIP (Hedgehog Interacting Protein) CLIA Kit (E-CL-M0379)

Name: Mouse HHIP (Hedgehog Interacting Protein) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0379
Price: 180.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Mouse HHIP (Hedgehog Interacting Protein) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse HHIP in samples.No significant cross-reactivity or interference between Mouse HHIP and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	58236.000	62856.000	60546.000	60513
1000	21951.000	26247.000	24099.000	24066
500	12853.000	11121.000	11987.000	11954
250	5382.000	6108.000	5745.000	5712
125	3011.000	2651.000	2831.000	2798
62.5	1373.000	1267.000	1320.000	1287
31.25	692.000	784.000	738.000	705
0	32.000	34.000	33.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	95.570	306.950	761.350	93.300	307.500	787.510
Standard deviation	11.980	32.570	68.450	10.480	28.230	64.420
CV（%）	12.540	10.610	8.990	11.230	9.180	8.180

Recovery

The recovery of Mouse HHIP was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	84-100	91
EDTA plasma （n=8）	86-98	93
Cell culture media （n=8）	89-100	94

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse HHIP and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	88-99	94-109	86-99
1:2	Average	93	101	93
1:4	Range	91-106	90-105	98-111
1:4	Average	99	97	104
1:8	Range	90-105	93-108	90-103
1:8	Average	98	99	96

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse HHIP. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HHIP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HHIP, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse HHIP. You can calculate the concentration of Mouse HHIP in the samples by comparing the RLU value of the samples to the standard curve.

Background