Mouse HSP-60 (Heat Shock Protein 60) CLIA Kit (E-CL-M0374)

Name: Mouse HSP-60 (Heat Shock Protein 60) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0374
Price: 180.0 USD
Availability: InStock

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Mouse HSP-60 (Heat Shock Protein 60) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse Hsp-60 in samples.No significant cross-reactivity or interference between Mouse Hsp-60 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	56730.000	59878.000	58304.000	58273
1000	25295.000	27383.000	26339.000	26308
500	12514.000	11566.000	12040.000	12009
250	5177.000	6047.000	5612.000	5581
125	2780.000	2470.000	2625.000	2594
62.5	1449.000	1417.000	1433.000	1402
31.25	647.000	741.000	694.000	663
0	30.000	32.000	31.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	99.220	241.580	939.430	96.720	264.770	974.560
Standard deviation	12.270	21.980	57.590	12.400	31.350	78.260
CV（%）	12.370	9.100	6.130	12.820	11.840	8.030

Recovery

The recovery of Mouse Hsp-60 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	90-104	97
EDTA plasma （n=8）	93-107	100
Cell culture media （n=8）	94-108	100

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse Hsp-60 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	88-103	100-118	93-105
1:2	Average	95	108	100
1:4	Range	94-110	86-102	86-101
1:4	Average	101	93	92
1:8	Range	83-95	93-107	92-107
1:8	Average	90	100	99

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse Hsp-60. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Hsp-60 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Hsp-60, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse Hsp-60. You can calculate the concentration of Mouse Hsp-60 in the samples by comparing the RLU value of the samples to the standard curve.

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