Mouse HSP-90 (Heat Shock Protein 90) CLIA Kit (E-CL-M0376)

Name: Mouse HSP-90 (Heat Shock Protein 90) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0376
Price: 180.0 USD
Availability: InStock

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Mouse HSP-90 (Heat Shock Protein 90) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse HSP-90 in samples.No significant cross-reactivity or interference between Mouse HSP-90 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	55647.000	63521.000	59584.000	59552
1000	22169.000	26459.000	24314.000	24282
500	11368.000	10814.000	11091.000	11059
250	5535.000	5837.000	5686.000	5654
125	2895.000	2619.000	2757.000	2725
62.5	1424.000	1394.000	1409.000	1377
31.25	689.000	703.000	696.000	664
0	31.000	33.000	32.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	90.960	316.140	837.490	87.780	323.200	825.430
Standard deviation	9.750	29.340	85.840	8.280	30.150	87.660
CV（%）	10.720	9.280	10.250	9.430	9.330	10.620

Recovery

The recovery of Mouse HSP-90 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	85-96	91
EDTA plasma （n=8）	89-102	96
Cell culture media （n=8）	100-115	105

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse HSP-90 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	88-101	102-114	93-108
1:2	Average	93	108	100
1:4	Range	98-116	90-104	89-101
1:4	Average	106	95	94
1:8	Range	95-111	87-100	95-107
1:8	Average	101	93	102

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse HSP-90. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HSP-90 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HSP-90, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse HSP-90. You can calculate the concentration of Mouse HSP-90 in the samples by comparing the RLU value of the samples to the standard curve.

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