Mouse IgE (Immunoglobulin E) CLIA Kit (E-CL-M0426)

Name: Mouse IgE (Immunoglobulin E) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0426
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Mouse IgE (Immunoglobulin E) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.09 ng/mL
Detection Range	0.16-10 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse IgE in samples.No significant cross-reactivity or interference between Mouse IgE and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
10	31359.000	36803.000	34081.000	34055
5	14650.000	16908.000	15779.000	15753
2.5	8476.000	7284.000	7880.000	7854
1.25	3648.000	4370.000	4009.000	3983
0.63	1878.000	1666.000	1772.000	1746
0.31	871.000	779.000	825.000	799
0.16	348.000	392.000	370.000	344
0	25.000	27.000	26.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	0.530	1.510	4.820	0.550	1.640	5.230
Standard deviation	0.060	0.160	0.310	0.070	0.120	0.450
CV（%）	11.320	10.600	6.430	12.730	7.320	8.600

Recovery

The recovery of Mouse IgE was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	89-102	94
EDTA plasma （n=8）	92-106	100
Cell culture media （n=8）	85-100	92

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse IgE and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	92-106	91-105	94-109
1:2	Average	98	96	101
1:4	Range	96-111	86-97	99-113
1:4	Average	103	91	107
1:8	Range	97-113	92-107	93-107
1:8	Average	105	98	99

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgE. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IgE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IgE, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse IgE. You can calculate the concentration of Mouse IgE in the samples by comparing the RLU value of the samples to the standard curve.

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