Mouse IgG2a (Immunoglobulin G2a) CLIA Kit (E-CL-M0429)

Name: Mouse IgG2a (Immunoglobulin G2a) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0429
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Mouse IgG2a (Immunoglobulin G2a) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.94 ng/mL
Detection Range	1.56-100 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse IgG2a in samples.No significant cross-reactivity or interference between Mouse IgG2a and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
100	35686.000	42288.000	38987.000	38950
50	16235.000	16263.000	16249.000	16212
25	8235.000	7413.000	7824.000	7787
12.5	3720.000	4144.000	3932.000	3895
6.25	2025.000	1905.000	1965.000	1928
3.13	1097.000	1069.000	1083.000	1046
1.56	607.000	633.000	620.000	583
0	37.000	37.000	37.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	4.580	14.980	41.520	4.550	14.380	40.610
Standard deviation	0.430	1.480	4.180	0.420	1.030	3.010
CV（%）	9.390	9.880	10.070	9.230	7.160	7.410

Recovery

The recovery of Mouse IgG2a was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	95-109	100
EDTA plasma （n=8）	91-105	96
Cell culture media （n=8）	101-115	106

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse IgG2a and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	90-103	88-101	95-110
1:2	Average	97	94	102
1:4	Range	92-104	101-119	103-116
1:4	Average	97	108	109
1:8	Range	88-101	93-108	87-103
1:8	Average	94	99	94

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgG2a. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IgG2a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IgG2a, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse IgG2a. You can calculate the concentration of Mouse IgG2a in the samples by comparing the RLU value of the samples to the standard curve.

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