Mouse IL-1α (Interleukin 1 Alpha) CLIA Kit (E-CL-M0034)

Name: Mouse IL-1α (Interleukin 1 Alpha) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0034
Price: 180.0 USD
Availability: InStock

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Mouse IL-1α (Interleukin 1 Alpha) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	4.69 pg/mL
Detection Range	7.81-500 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse IL-1α in samples.No significant cross-reactivity or interference between Mouse IL-1α and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
500	53060.000	54126.000	53593.000	53568
250	20756.000	21680.000	21218.000	21193
125	10083.000	8733.000	9408.000	9383
62.5	4683.000	4717.000	4700.000	4675
31.25	2423.000	2319.000	2371.000	2346
15.63	1336.000	1218.000	1277.000	1252
7.81	764.000	784.000	774.000	749
0	24.000	26.000	25.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	26.500	48.550	210.790	28.100	46.530	214.550
Standard deviation	2.340	4.900	16.100	3.300	4.040	13.130
CV（%）	8.830	10.090	7.640	11.740	8.680	6.120

Recovery

The recovery of Mouse IL-1α was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	88-101	95
EDTA plasma （n=8）	92-106	99
Cell culture media （n=8）	90-103	95

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse IL-1α and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	85-99	94-109	101-115
1:2	Average	92	101	107
1:4	Range	83-97	95-107	86-101
1:4	Average	90	102	93
1:8	Range	89-104	95-106	98-113
1:8	Average	96	100	104

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-1α. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse IL-1α. You can calculate the concentration of Mouse IL-1α in the samples by comparing the RLU value of the samples to the standard curve.

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