Mouse IL-13 (Interleukin 13) CLIA Kit (E-CL-M0460)

Name: Mouse IL-13 (Interleukin 13) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0460
Price: 180.0 USD
Availability: InStock

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Mouse IL-13 (Interleukin 13) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	7.5 pg/mL
Detection Range	12.5-800 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse IL-13 in samples.No significant cross-reactivity or interference between Mouse IL-13 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
800	57466.000	58028.000	57747.000	57709
400	22810.000	24314.000	23562.000	23524
200	10355.000	10189.000	10272.000	10234
100	4807.000	4981.000	4894.000	4856
50	2353.000	2053.000	2203.000	2165
25	1106.000	1066.000	1086.000	1048
12.5	483.000	535.000	509.000	471
0	38.000	38.000	38.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	43.380	128.320	267.480	43.070	128.030	275.460
Standard deviation	4.530	12.020	28.970	4.910	14.340	28.370
CV（%）	10.440	9.370	10.830	11.400	11.200	10.300

Recovery

The recovery of Mouse IL-13 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	91-105	98
EDTA plasma （n=8）	92-107	100
Cell culture media （n=8）	95-110	102

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse IL-13 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	86-100	94-109	100-112
1:2	Average	93	101	106
1:4	Range	84-98	97-114	92-104
1:4	Average	91	104	97
1:8	Range	87-100	86-99	103-115
1:8	Average	93	91	109

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-13. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-13, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse IL-13. You can calculate the concentration of Mouse IL-13 in the samples by comparing the RLU value of the samples to the standard curve.

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