Mouse IL-21 (Interleukin 21) CLIA Kit (E-CL-M0693)

Name: Mouse IL-21 (Interleukin 21) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0693
Price: 180.0 USD
Availability: InStock

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Mouse IL-21 (Interleukin 21) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	7.5 pg/mL
Detection Range	12.5-800 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse IL-21 in samples.No significant cross-reactivity or interference between Mouse IL-21 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
800	53096.000	57920.000	55508.000	55477
400	22321.000	24983.000	23652.000	23621
200	10455.000	10181.000	10318.000	10287
100	4531.000	5337.000	4934.000	4903
50	2298.000	2176.000	2237.000	2206
25	1163.000	1017.000	1090.000	1059
12.5	512.000	560.000	536.000	505
0	31.000	31.000	31.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	40.590	125.370	361.740	42.000	113.280	333.340
Standard deviation	5.100	13.880	26.010	4.630	13.530	38.230
CV（%）	12.560	11.070	7.190	11.020	11.940	11.470

Recovery

The recovery of Mouse IL-21 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	88-100	93
EDTA plasma （n=8）	85-95	90
Cell culture media （n=8）	100-118	108

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse IL-21 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	96-108	94-108	87-101
1:2	Average	102	99	94
1:4	Range	100-116	102-118	91-106
1:4	Average	106	109	96
1:8	Range	97-109	102-115	94-105
1:8	Average	104	108	100

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-21. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-21 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-21, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse IL-21. You can calculate the concentration of Mouse IL-21 in the samples by comparing the RLU value of the samples to the standard curve.

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