Mouse INS-Ab(Insulin Autoantibody) CLIA Kit (E-CL-M0436)
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For research use only.
Product Summary
| Sensitivity | 18.75 pg/mL |
| Detection Range | 31.25-2000 pg/mL |
| Sample Volume | 100 μL |
| Total Assay Time | 4 h 30 min |
| Reactivity | Mouse |
| Specificity | This kit recognizes Mouse IAA in samples.No significant cross-reactivity or interference between Mouse IAA and analogues was observed |
| Recovery | 80%-120% |
| Sample Type | Serum, plasma and other biological fluids |
| Detection Method | Chemiluminescence |
| Assay Type | Sandwich-CLIA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃/-20℃ |
| Expiration Date | 6 months |
Performance
Test Principle
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IAA. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IAA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IAA, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse IAA. You can calculate the concentration of Mouse IAA in the samples by comparing the RLU value of the samples to the standard curve.
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