Mouse INSR-β (Insulin Receptor Beta Subunit) CLIA Kit (E-CL-M0438)

Name: Mouse INSR-β (Insulin Receptor Beta Subunit) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0438
Price: 180.0 USD
Availability: InStock

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Mouse INSR-β (Insulin Receptor Beta Subunit) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.19 ng/mL
Detection Range	0.31-20 ng/mL
Sample Volume	100 μL
Total Assay Time	4 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse INSR-β in samples.No significant cross-reactivity or interference between Mouse INSR-β and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
20	31871.000	37139.000	34505.000	34479
10	12719.000	14069.000	13394.000	13368
5	5688.000	5028.000	5358.000	5332
2.5	2561.000	2651.000	2606.000	2580
1.25	1565.000	1391.000	1478.000	1452
0.63	996.000	904.000	950.000	924
0.31	637.000	695.000	666.000	640
0	25.000	27.000	26.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	0.920	2.690	8.630	0.940	2.750	9.430
Standard deviation	0.090	0.270	0.630	0.120	0.270	0.650
CV（%）	9.780	10.040	7.300	12.770	9.820	6.890

Recovery

The recovery of Mouse INSR-β was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	96-108	101
EDTA plasma （n=8）	92-109	99
Cell culture media （n=8）	99-113	105

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse INSR-β and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	96-109	89-102	88-103
1:2	Average	103	95	95
1:4	Range	89-104	85-99	97-114
1:4	Average	96	92	104
1:8	Range	92-105	100-115	87-97
1:8	Average	100	107	92

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse INSR-β. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse INSR-β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse INSR-β, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse INSR-β. You can calculate the concentration of Mouse INSR-β in the samples by comparing the RLU value of the samples to the standard curve.