Mouse LH (Luteinizing Hormone) CLIA Kit (E-CL-M0053)

Name: Mouse LH (Luteinizing Hormone) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0053
Price: 180.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Mouse LH (Luteinizing Hormone) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	0.19 mIU/mL
Detection Range	0.31-20 mIU/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse LH in samples.No significant cross-reactivity or interference between Mouse LH and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

mIU/mL	OD1	OD2	Mean OD	Corrected OD
20	33311.000	39103.000	36207.000	36173
10	12601.000	14027.000	13314.000	13280
5	5790.000	5532.000	5661.000	5627
2.5	2414.000	2804.000	2609.000	2575
1.25	1441.000	1405.000	1423.000	1389
0.63	933.000	859.000	896.000	862
0.31	630.000	672.000	651.000	617
0	34.000	34.000	34.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（mIU/mL）	0.960	2.370	7.490	0.870	2.250	7.840
Standard deviation	0.080	0.190	0.680	0.070	0.240	0.610
CV（%）	8.330	8.020	9.080	8.050	10.670	7.780

Recovery

The recovery of Mouse LH was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	95-109	102
EDTA plasma （n=8）	87-100	94
Cell culture media （n=8）	93-107	99

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse LH and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	103-117	95-109	91-103
1:2	Average	109	101	98
1:4	Range	96-112	88-100	94-111
1:4	Average	102	95	102
1:8	Range	92-108	89-102	102-118
1:8	Average	98	94	107

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse LH. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LH, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse LH. You can calculate the concentration of Mouse LH in the samples by comparing the RLU value of the samples to the standard curve.

Background