Mouse MCP-1 (Monocyte Chemotactic Protein 1) CLIA Kit (E-CL-M0006)

Name: Mouse MCP-1 (Monocyte Chemotactic Protein 1) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0006
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Mouse MCP-1 (Monocyte Chemotactic Protein 1) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	4.69 pg/mL
Detection Range	7.81-500 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse MCP-1 in samples.No significant cross-reactivity or interference between Mouse MCP-1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
500	53342.000	60038.000	56690.000	56665
250	20872.000	21578.000	21225.000	21200
125	9852.000	9016.000	9434.000	9409
62.5	4501.000	5053.000	4777.000	4752
31.25	2640.000	2274.000	2457.000	2432
15.63	1370.000	1288.000	1329.000	1304
7.81	794.000	828.000	811.000	786
0	25.000	25.000	25.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	23.420	46.950	200.650	22.290	50.440	219.520
Standard deviation	2.460	4.000	13.000	2.490	4.230	22.920
CV（%）	10.500	8.520	6.480	11.170	8.390	10.440

Recovery

The recovery of Mouse MCP-1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	91-104	97
EDTA plasma （n=8）	93-107	100
Cell culture media （n=8）	101-116	108

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse MCP-1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	102-118	91-105	90-104
1:2	Average	110	98	98
1:4	Range	87-100	89-105	96-109
1:4	Average	92	96	102
1:8	Range	86-99	94-108	86-98
1:8	Average	92	100	91

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse MCP-1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MCP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MCP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse MCP-1. You can calculate the concentration of Mouse MCP-1 in the samples by comparing the RLU value of the samples to the standard curve.

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