Mouse MMP-7 (Matrix Metalloproteinase 7) CLIA Kit (E-CL-M0494)

Name: Mouse MMP-7 (Matrix Metalloproteinase 7) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0494
Price: 180.0 USD
Availability: InStock

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Mouse MMP-7 (Matrix Metalloproteinase 7) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse MMP-7 in samples.No significant cross-reactivity or interference between Mouse MMP-7 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	54973.000	62417.000	58695.000	58672
500	22765.000	24547.000	23656.000	23633
250	10955.000	10379.000	10667.000	10644
125	5003.000	5355.000	5179.000	5156
62.5	2645.000	2299.000	2472.000	2449
31.25	1391.000	1291.000	1341.000	1318
15.63	731.000	763.000	747.000	724
0	22.000	24.000	23.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	47.020	123.690	416.570	46.550	133.750	427.380
Standard deviation	6.030	8.960	37.820	4.230	11.810	46.030
CV（%）	12.820	7.240	9.080	9.090	8.830	10.770

Recovery

The recovery of Mouse MMP-7 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	88-99	93
EDTA plasma （n=8）	86-99	93
Cell culture media （n=8）	94-110	100

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse MMP-7 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	91-102	97-112	87-100
1:2	Average	97	103	94
1:4	Range	92-104	102-115	90-103
1:4	Average	98	108	97
1:8	Range	99-112	92-107	101-120
1:8	Average	105	98	109

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse MMP-7. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MMP-7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MMP-7, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse MMP-7. You can calculate the concentration of Mouse MMP-7 in the samples by comparing the RLU value of the samples to the standard curve.

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