Mouse NGAL (Neutrophil Gelatinase Associated Lipocalin) CLIA Kit (E-CL-M0514)

Name: Mouse NGAL (Neutrophil Gelatinase Associated Lipocalin) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0514
Price: 180.0 USD
Availability: InStock

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Mouse NGAL (Neutrophil Gelatinase Associated Lipocalin) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse NGAL in samples.No significant cross-reactivity or interference between Mouse NGAL and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	50878.000	61064.000	55971.000	55942
500	24010.000	24966.000	24488.000	24459
250	10988.000	10892.000	10940.000	10911
125	4968.000	5240.000	5104.000	5075
62.5	2723.000	2629.000	2676.000	2647
31.25	1439.000	1323.000	1381.000	1352
15.63	717.000	719.000	718.000	689
0	28.000	30.000	29.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	47.930	122.000	435.640	44.280	125.610	472.250
Standard deviation	5.390	11.990	37.330	4.680	14.990	49.350
CV（%）	11.250	9.830	8.570	10.570	11.930	10.450

Recovery

The recovery of Mouse NGAL was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	92-106	98
EDTA plasma （n=8）	100-114	106
Cell culture media （n=8）	91-104	97

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse NGAL and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	85-98	96-113	101-115
1:2	Average	91	104	109
1:4	Range	86-98	104-119	86-100
1:4	Average	93	110	92
1:8	Range	86-100	94-109	103-117
1:8	Average	92	100	109

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse NGAL. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NGAL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NGAL, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse NGAL. You can calculate the concentration of Mouse NGAL in the samples by comparing the RLU value of the samples to the standard curve.

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