Mouse PRL (Prolactin) CLIA Kit (E-CL-M0070)

Name: Mouse PRL (Prolactin) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0070
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse PRL in samples.No significant cross-reactivity or interference between Mouse PRL and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	57005.000	60199.000	58602.000	58568
1000	23496.000	24718.000	24107.000	24073
500	11528.000	10548.000	11038.000	11004
250	5681.000	5931.000	5806.000	5772
125	2935.000	2627.000	2781.000	2747
62.5	1356.000	1270.000	1313.000	1279
31.25	709.000	739.000	724.000	690
0	34.000	34.000	34.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	104.900	303.110	732.760	112.340	317.730	784.070
Standard deviation	8.510	33.490	80.600	10.720	32.920	56.920
CV（%）	8.110	11.050	11.000	9.540	10.360	7.260

Recovery

The recovery of Mouse PRL was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	89-101	95
EDTA plasma （n=8）	99-116	107
Cell culture media （n=8）	86-98	93

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse PRL and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	91-107	91-105	86-100
1:2	Average	99	99	93
1:4	Range	100-119	89-104	94-112
1:4	Average	108	95	102
1:8	Range	87-102	83-95	93-107
1:8	Average	94	90	100

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PRL. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PRL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PRL, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse PRL. You can calculate the concentration of Mouse PRL in the samples by comparing the RLU value of the samples to the standard curve.

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