Mouse S100A10 (S100 Calcium-binding Protein A10) CLIA Kit (E-CL-M0701)

Name: Mouse S100A10 (S100 Calcium-binding Protein A10) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0701
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Mouse S100A10 (S100 Calcium-binding Protein A10) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse S100A10 in samples.No significant cross-reactivity or interference between Mouse S100A10 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	62184.000	64362.000	63273.000	63242
2000	27401.000	31307.000	29354.000	29323
1000	15245.000	13229.000	14237.000	14206
500	6731.000	7423.000	7077.000	7046
250	3632.000	3194.000	3413.000	3382
125	1694.000	1644.000	1669.000	1638
62.5	749.000	815.000	782.000	751
0	31.000	31.000	31.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	190.630	422.910	1639.010	176.350	395.600	1698.690
Standard deviation	23.180	45.970	170.620	17.480	36.200	190.420
CV（%）	12.160	10.870	10.410	9.910	9.150	11.210

Recovery

The recovery of Mouse S100A10 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	91-107	99
EDTA plasma （n=8）	90-102	97
Cell culture media （n=8）	94-111	102

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse S100A10 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	89-102	92-107	99-113
1:2	Average	95	99	106
1:4	Range	96-109	87-98	100-116
1:4	Average	103	92	108
1:8	Range	96-110	91-104	101-117
1:8	Average	102	98	108

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse S100A10. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse S100A10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse S100A10, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse S100A10. You can calculate the concentration of Mouse S100A10 in the samples by comparing the RLU value of the samples to the standard curve.

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