Mouse S100A9 (S100 Calcium Binding Protein A9) CLIA Kit (E-CL-M0700)

Name: Mouse S100A9 (S100 Calcium Binding Protein A9) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0700
Price: 180.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Mouse S100A9 (S100 Calcium Binding Protein A9) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse S100A9 in samples.No significant cross-reactivity or interference between Mouse S100A9 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	54212.000	58818.000	56515.000	56475
1000	24414.000	25240.000	24827.000	24787
500	11878.000	11508.000	11693.000	11653
250	5269.000	5815.000	5542.000	5502
125	2834.000	2500.000	2667.000	2627
62.5	1357.000	1287.000	1322.000	1282
31.25	685.000	699.000	692.000	652
0	38.000	42.000	40.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	97.120	313.460	998.060	99.660	287.410	1040.150
Standard deviation	8.050	25.420	83.040	11.720	25.610	66.990
CV（%）	8.290	8.110	8.320	11.760	8.910	6.440

Recovery

The recovery of Mouse S100A9 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	90-106	96
EDTA plasma （n=8）	83-98	90
Cell culture media （n=8）	93-105	98

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse S100A9 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	92-106	95-109	86-97
1:2	Average	97	101	92
1:4	Range	91-103	84-99	91-105
1:4	Average	97	91	99
1:8	Range	86-100	89-101	92-104
1:8	Average	91	94	97

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse S100A9. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse S100A9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse S100A9, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse S100A9. You can calculate the concentration of Mouse S100A9 in the samples by comparing the RLU value of the samples to the standard curve.

Background