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Mouse TAT(Thrombin/Antithrombin Complex) ELISA Kit (E-EL-M1138)

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All Size Price Qty
96T $ 609.00
48T $ 487.00
24T $ 150.00
96T*5 Inquire /
96T*10 Inquire /
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For research use only.

Product Summary
Sensitivity 9.38 pg/mL
Detection Range 15.63-1000 pg/mL
Sample Volume 100 μL
Total Assay Time 3 h 30 min
Reacitivity Mouse
Specificity This kit recognizes Mouse TAT in samples. No significant cross-reactivity or interference between Mouse TAT and analogues was observed
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Sandwich
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃/-20℃
Expiration Date 12 months
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TAT. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TAT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TAT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TAT. You can calculate the concentration of Mouse TAT in the samples by comparing the OD of the samples to the standard curve.
Thrombin‐antithrombin complex is a molecular complex composed of thrombin and AT, a primary thrombin inhibitor, in a 1:1 ratio. Increased TAT indicates excess thrombin production and serve as a marker reflecting prothrombotic status. Increased thrombin levels signify activation of the blood coagulation cascade. However, thrombin levels cannot be measured directly due to extremely short half‐life in blood, whereas TAT has a half‐life of 3‐15 minutes, enabling direct measurement. Consequently, TAT could be used to indirectly assess thrombin generation as a molecular marker of activated coagulation and reflect ongoing condition of DIC
Research Area Cancer , Cell Biology , Cardiovascular , Signal Transduction
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