Mouse TAT(Thrombin/Antithrombin Complex) ELISA Kit (E-EL-M1138)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TAT. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TAT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TAT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TAT. You can calculate the concentration of Mouse TAT in the samples by comparing the OD of the samples to the standard curve.

Thrombin‐antithrombin complex is a molecular complex composed of thrombin and AT, a primary thrombin inhibitor, in a 1:1 ratio. Increased TAT indicates excess thrombin production and serve as a marker reflecting prothrombotic status. Increased thrombin levels signify activation of the blood coagulation cascade. However, thrombin levels cannot be measured directly due to extremely short half‐life in blood, whereas TAT has a half‐life of 3‐15 minutes, enabling direct measurement. Consequently, TAT could be used to indirectly assess thrombin generation as a molecular marker of activated coagulation and reflect ongoing condition of DIC