Mouse TNF-α (Tumor Necrosis Factor Alpha) CLIA Kit (E-CL-M0047)

Name: Mouse TNF-α (Tumor Necrosis Factor Alpha) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0047
Price: 180.0 USD
Availability: InStock

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Mouse TNF-α (Tumor Necrosis Factor Alpha) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	7.5 pg/mL
Detection Range	12.5-800 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse TNF-α in samples.No significant cross-reactivity or interference between Mouse TNF-α and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
800	55957.000	57251.000	56604.000	56574
400	21810.000	25276.000	23543.000	23513
200	11101.000	9653.000	10377.000	10347
100	4581.000	5157.000	4869.000	4839
50	2224.000	2214.000	2219.000	2189
25	1171.000	1089.000	1130.000	1100
12.5	483.000	553.000	518.000	488
0	29.000	31.000	30.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	42.220	117.240	278.120	41.210	125.300	276.400
Standard deviation	5.190	11.060	27.450	4.520	11.160	26.700
CV（%）	12.290	9.430	9.870	10.970	8.910	9.660

Recovery

The recovery of Mouse TNF-α was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	96-112	103
EDTA plasma （n=8）	88-100	93
Cell culture media （n=8）	86-98	93

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse TNF-α and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	96-114	98-115	101-119
1:2	Average	104	106	109
1:4	Range	92-103	93-105	88-104
1:4	Average	97	100	95
1:8	Range	86-100	89-102	99-113
1:8	Average	92	95	105

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TNF-α. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TNF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TNF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse TNF-α. You can calculate the concentration of Mouse TNF-α in the samples by comparing the RLU value of the samples to the standard curve.

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