Mouse VEGF-D (Vascular Endothelial cell Growth Factor D) CLIA Kit (E-CL-M0680)

Name: Mouse VEGF-D (Vascular Endothelial cell Growth Factor D) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0680
Price: 180.0 USD
Availability: InStock

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Mouse VEGF-D (Vascular Endothelial cell Growth Factor D) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	7.5 pg/mL
Detection Range	12.5-800 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse VEGF-D in samples.No significant cross-reactivity or interference between Mouse VEGF-D and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
800	51756.000	62128.000	56942.000	56909
400	20730.000	24118.000	22424.000	22391
200	10723.000	10211.000	10467.000	10434
100	4725.000	4759.000	4742.000	4709
50	2245.000	2211.000	2228.000	2195
25	1230.000	1048.000	1139.000	1106
12.5	538.000	560.000	549.000	516
0	32.000	34.000	33.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	36.570	70.700	336.350	36.700	67.990	316.300
Standard deviation	3.550	6.820	26.540	3.770	6.540	33.720
CV（%）	9.710	9.650	7.890	10.270	9.620	10.660

Recovery

The recovery of Mouse VEGF-D was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	90-104	95
EDTA plasma （n=8）	99-113	105
Cell culture media （n=8）	93-106	100

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse VEGF-D and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	103-118	102-119	92-104
1:2	Average	109	109	99
1:4	Range	88-100	101-117	102-117
1:4	Average	95	108	108
1:8	Range	91-105	98-111	92-104
1:8	Average	98	103	97

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse VEGF-D. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse VEGF-D and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse VEGF-D, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse VEGF-D. You can calculate the concentration of Mouse VEGF-D in the samples by comparing the RLU value of the samples to the standard curve.

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