Oxalate Colorimetric Assay Kit (E-BC-K892-S)
For research use only.
| Detection Principle | Oxalic acid is a simple organic acid in organisms and has important physiological functions. The detection principle of this kit: Oxalate oxidase catalyzes the oxidation of oxalate to produce hydrogen peroxide and carbon dioxide. Under the action of HRP, hydrogen peroxide reacts with chromogenic substances to produce colored products. There is a maximum absorption peak at 550 nm, and the absorbance is proportional to the content of oxalate. |
| Sample Type | Serum,Plasma,Urine,Plant and animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (550 nm) |
| Research Area | Liver And Renal Function , Plant Physiology Series |
| Other Reagents Required | Absolute ethanol |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.08 mmol/L |
| Detection Range | 0.08-1.00 mmol/L |
| Precision | inter-assay CV: 1.8-2.05% | intra-assay CV: 4.5-7.2 % |
| Sample Volume | 100 μL |
| Assay Time | 50 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Rats plasma | 1 |
| Human plasma | 1 |
| Human urine | 1 |
| 10% Epipremnum aureum tissue homogenate | 2-3 |
| 10% Spinach tissue homogenate | 1 |
| 10% Pumpkin tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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